Biography
Professor Lawrence Abraham completed his Bachelor of Science
with 1st class Honours at Griffith University in Brisbane. He
then studied for his PhD at Murdoch University, in Perth, and
Monash University, in Melbourne. Prof Abraham undertook post
doctoral research at the Scripps Research Institute in San Diego
before being awarded a Senior Research Fellowship at Royal Perth
Hospital in Clinical Immunology. In 1994 he moved to the
University of Western Australia as a lecturer. Prof Abraham is
also an Adjunct member of the Southwest Foundation for Biomedical
Research, in San Antonio, Texas, USA.
Research Interests
Our group is interested in the transcriptional
regulation of gene expression. Our focus is on genes that are
involved in regulating the immune and inflammatory responses and
the identification of transcription factors that regulate these
genes. Our long-term aim is to develop therapeutic strategies to
modulate the activity of these genes in order to prevent disease.
We are also interested in the effects of genetic polymorphism
(inc. SNPs) on the expression of genes, particularly promoter and
other regulatory variants. More information on Prof Abraham's Research
Techniques and Expertise
Molecular biology: DNA & RNA
purification, DNA cloning, library screening, Southern, Northern
and Western blotting, site-directed mutagenesis, DNase I
footprinting, electrophoretic mobility shift assays, in situ
hybridisation, Chromatin Immunoprecipation, Chromatin mapping by
nuclease digestion, DNA sequencing, quantitative PCR, yeast
one-hybrid system.
Cell biology: Tissue culture, Transient
transfections, luciferase assays, immunocytochemistry, flow
cytometry, FACS.
Biochemistry: Protein purification, gel
filtration, affinity purification (glutathione/DNA), peptide mass
fingerprinting, SDS-PAGE.
Molecular Genetics: Regulatory SNP
detection, Microsatellite typing, Allele-specific DNA sequencing,
bioinformatics, genome analysis, touch-down PCR, genetic
association & linkage analysis, 2D - Proteomics.
Students
Desire Ho (PhD) CD30 gene regulation in anaplastic large cell
lymphoma
Hui Min Cheah (Hons) SNP variation in heart disease
Luke Diepeveen (Hons) SNP variation in cysitnosis
Denise Schutze (MSc exchange student) CD30 signaling in
cancer
Collaborators
Dr Eric Moses, Southwest Foundation for Biomedical Research,
San Antonio
Dr John Blangero, Southwest Foundation for Biomedical Research,
San Antonio
Dr Jeremy Jowett, Baker IDI Heart & Diabetes Institute,
Melbourne
Dr Scott Stewart, Chemistry, UWA
Dr Daniela Ulgiati, Biochemistry & Molecular Biology, UWA
Memberships
Australian Society of Biochemistry and Molecular Biology
American Society of Biochemistry and Molecular Biology
International Cytokine Society
Australasian Society for Immunology
Funding
National Health & Medical Research Council
Cancer Council, Australia
Australian Heart Foundation
Medical & Health Research Infrastructure Fund
Teaching
GENE2204 Principles of Genetics
(Unit Co-ordinator)
GENE3330 Molecular Genetics and Genomics
GENE2230 Molecular Genetics
SCIE2225 Molecular Biology
SCIE3325 Molecular Biology
BIOC3351 Molecular and Structural Biochemistry
BIOC3352 Cellular and Metabolic Biochemistry
|
